Other Services
Interested parties can contact assistant core director to discuss preliminary approach of intended creation of a mouse model. Primary requirement to generate a mouse model at BCH using mouse core services is the PI must have an approved IACUC and IBC protocol for the desired model. After approval is granted the mouse core will co-ordinate with PI or lab member to submit request form and provide required reagents before scheduling an experiment.
Requirements
- Approved IACUC and IBC protocol for the desired mouse model.
- Submit electronic request form (eForm) for services signed by PI and designated lab member.
- Mouse core will co-ordinate with PI or lab member to collect required reagents before scheduling an experiment.
- A fund number or purchase order number to reimburse fees for requested services.
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Southern Blot Service
Core provide Southern blot analysis in following categories:
- Tail DNA samples
- ES cell DNA samples
DNA samples digested using restriction enzyme/s overnight (18-20 hours) followed by DNA fragment separation on agarose gel. Gel is denatured and neutralized and capillary transferred to HyBond (Amersham) Nylon membrane for 18-20 hours. Membrane is UV-crosslinked and prehybridized at 65oC o/n. Probe DNA is labelled using 32P-dCTP and DNA labelling kit. Prehybridized membrane is hybridized with the labelled probe at 65oC 18-20 hours, washed with buffers to eliminate background and exposed to X-Ray film.
Mycoplasma Testing Service
This service is outsourced, please contact for details.