Quicklinks
NOTE: All forms must be submitted electronically through this website, and will not be accepted in printed or scanned format. Only completed/signed forms will be processed.
Overview
We alter mouse genomes either by utilizing genome editing CRISPR/Cas9 reagents, random insertion of transgenic DNA or gene targeting techniques in mouse embryonic stem cells. The Core also provides services towards the identification, propagation and management of founder mouse lines, cryopreservation, re-derivation and development of mouse-oriented research projects in the IDDRC and Boston Children’s Hospital scientific community.
The Core consists of a Director, an Assistant Director and trained staff members. The assistant director oversees daily operation including expert consultation, quality control of regents, monitor progress of each project, communicating with the PI and lab members, trouble shooting, scheduling, billing and direct supervision of staff members.
Cite the Core
Please cite the Gene Manipulation Core in your publications in the following way:
We thank the IDDRC Gene Manipulation Core, funded by NIH P50 HD105351.
Gene Manipulations
Contact Us
Core Services & Fees
(These prices are effective October 1, 2023 – September 30, 2024. Please contact the core for industry pricing)
| Pronuclear Injection Services | IDDRC | BCH | Non-BCH/Non-IDDRC |
| CRISPR/Cas9 – Pronuclear Injection/Session | $4,620 | $5,082 | $5,544* |
| Transgenic DNA injection (<20kb)/Construct using B6 donor | $5,216 | $5,738 | $6,259 |
| Transgenic BAC DNA injection (<300kb)/Construct using B6 donor | $5,216 | $5,738 | $6,259 |
| Microinjection | IDDRC | BCH | Non-BCH/Non-IDDRC |
| Blastocyst injection – per ES cell line using B6 donors | $4,146 | $4,561 | $4,975 |
Microinjection > Get CRISPR Injection Form > Get Transgenic DNA Injection Form >
| Cryopreservation Services | IDDRC | BCH | Non-BCH/Non-IDDRC |
| Embryo Cryopreservation (Cryo-1) -100-150 embryos | $2,070 | $2,168 | $2,365 |
| Embryo Cryopreservation (Cryo-2)- 150-300 embryos | $2,920 | $3,059 | $3,337 |
| Embryo Cryopreservation (Cryo-3)- 300-450 embryos | $4,103 | $4,078.8 | $4,923.6 |
| Sperm Cryopreservation up to 3 males | $1,428 | $1,571 | NA |
| Cryopreserved Embryo/Sperm storage Fees/Line/Year | $178 | $196 | $214 |
Cryopreservation > Get Embryo Form > Get Sperm Form > Get Storage Form >
| Mouse ES Cell Services | IDDRC | BCH | Non-BCH/Non-IDDRC |
| ES cell line derivation up to 6 cell lines | $2,225 | $2,248 | $2,670 |
| Gene targeting – homologous recombination (up to 192 clones) | $5,733 | $6,306 | $6,880 |
| Gene targeting – homologous recombination (up to 384 clones) | $9,466 | $10,413 | $11,359 |
| Cre or Flpe deletion in vitro (up to 96 clones) | $2,867 | $3,154 | $3,440 |
| Karyotyping ES cells/cell line | $550 | $408 | $445 |
| CRISPR/Cas9 Gene targeting in mouse ES cell (up to 192 clones) | $4,620 | $5,206 | $5,680 * |
| ES clone DNA extraction from 6 Well | TBD | TBD | TBD |
| Rederivation Services | IDDRC | BCH | Non-BCH/Non-IDDRC |
| Re-derivation by embryo transfer – Imported live mice within ARCH | $1,596 | $1,713 | $1,915 |
| Re-derivation- Serology tests – sample collection by ARCH personnel | TBD | TBD | TBD |
| Re-derivation- embryo transfer (frozen embryos 2-8 cells) | $1,557 | $1,713 | $1,868 |
| In vitro fertilization/session | $2,336 | $2,570 | $2,803 |
| DNA Purification Services | IDDRC | BCH | Non-BCH/Non-IDDRC |
| Transgenic DNA purification | $488 | $488 | $533 |
| BAC DNA preparation | $649 | $714 | $779 |
| BAC DNA digestion & CL4B column purification | $649 | $714 | $779 |
| ES clone DNA extraction- 96W | $550 | $567 | $618 |
| ES clone expansion for injection or frozen stock/clone | $311 | $342 | $373 |
| Mouse Tail Biopsy | $6.50 | $7.15 | $7.8 |
| Mouse Tail DNA prep | $6.50 | $7 | $8 |
| Other Services | IDDRC | BCH | Non-BCH/Non-IDDRC |
| Southern blot analysis/Gel/Probe | $2,750 | $3,025 | $3,300 |
| Mycoplasma testing (Outsourced through ARCH) Please contact for details > | TBD | TBD | TBD |
| Tech Time hourly rates | $78 | $86 | $94 |
*CRISPR license fee will be added to these services