Gene Editing Group

Quicklinks

Transgenic DNA Injection Form > CRISPR Injection Form > ES Microinjection Form > Rederivation Form >

Embryo Cryopreservation Form > Sperm Cryopreservation Form > Cryopreservation Storage Form > Gene Targeting Form >

NOTE: All forms must be submitted electronically through this website, and will not be accepted in printed or scanned format. Only completed/signed forms will be processed.

Overview

The Gene Editing Group Facility works with investigators to create genetically engineered mouse models. Our goal is to offer expert advice and technical expertise in an easily accessible, affordable and efficient manner.

We alter mouse genomes either by utilizing genome editing CRISPR/Cas9 reagents, random insertion of transgenic DNA or gene targeting techniques in mouse embryonic stem cells. The Core also provides services towards the identification, propagation and management of founder mouse lines, cryopreservation, re-derivation and development of mouse-oriented research projects in the IDDRC and Boston Children’s Hospital scientific community.

The Core consists of a Director, an Assistant Director and trained staff members. The assistant director oversees daily operation including expert consultation, quality control of regents, monitor progress of each project, communicating with the PI and lab members, trouble shooting, scheduling, billing and direct supervision of staff members.

Cite the Core

Please cite the Gene Manipulation Core in your publications in the following way:
We thank the IDDRC Gene Manipulation Core, funded by NIH P50 HD105351.