Animal Behavior & Physiology Core

Overview

The Animal Behavior and Physiology (AB&P) Core is designed to provide a comprehensive state-of-the-art in vivo preclinical, translational platform for in-depth phenotyping of models of neurodevelopmental disorders, development of reliable and quantitative biomarkers, and to measure the effects of therapeutic interventions. The AB&P leadership and expanded research team includes expertise in translational neuroscience, rodent behavioral and physiological metrics, drug testing, biomedical engineering, and in core management to facilitate efficient planning, execution and analyses of experimental data.

We recognize the need to enhance and integrate multilevel phenotyping capabilities used for characterizing our animal models both at the behavioral and physiological level at Boston Children’s Hospital within one rodent phenotyping core. The AB&P has been serving investigators since 2011 and has contributed to the publication of over 50 manuscripts in peer-reviewed journals and provided facilities for scientists to obtain data to support numerous successful grant applications. In 2019, the AB&P was recognized for excellence and broad appeal across multiple domains by being made an Institutional Core Facility at Boston Children’s.

Cite the Core

Please cite the Animal Behavior and Physiology Core in your publications in the following way:
We thank the IDDRC Animal Behavior and Physiology Core, funded by NIH P50 HD105351

AB&P Advanced Technologies

The AB&P facilities include a wide range of advanced technologies for acquiring data at single cell, network and the behavioral level. Such technologies can be combined in the majority of cases in conscious freely behaving rodents, allowing top-down and bottom-up analysis. By design, each available technology is modular with capacity for upgrade, thus minimizing risk of obsolescence in coming years. The behavioral and physiological assays of the AB&P are designed to enable deep model phenotyping, and early phase CNS drug discovery and development by collection and analysis of in vivo behavioral and physiological signals. Services that provide a high-throughput platform for preclinical neurophysiological monitoring to support translational research are absent within the greater Boston area (inclusive of industry facilities). The AB&P thus addresses a conspicuous, unmet need and fosters Boston Children’s collaboration with industry and academia both in the Boston area and nationally.

As itemized on the equipment page, the equipment includes unique and modular platforms that will enable multi-layered, comprehensive neurodevelopmental disease model phenotyping. As well, these assays provide measures of target engagement by pharmacological agents and enable necessary preclinical PK/PD studies once a therapeutic is deployed in any of the available models.

Our Offerings

Specifically, the unique features of the AB&P Core include:

  • ability to house mice in customized modified environments
  • a 24-cage wireless video telemetry setup for continuous recording of EEG, EMG, EKG, actigraphy, body temperature and other metrics
  • a setup for rodent transcranial magnetic stimulation (TMS)
  • in vivo calcium imaging by multifiber photometry coupled with behavioral analysis such as operant behavior, social or anxiety tests,
  • in vivo microdialysis for sampling neurochemicals and other constituents of the peripheral and central nervous systems in a conscious, freely moving rodent.

Notably, AB&P measures are by design translational, and available in human studies at Boston Children’s for immediate extension of hypotheses formulated in the preclinical AB&P studies to clinical trials.

Contact Us

Co-Directors

Michela Fagiolini, PhD
Alex Rotenberg, MD, PhD

Assistant Director, Behavior
Nathaniel Hodgson, PhD
Email >

Assistant Director, Physiology
Mustafa Hameed, MD
Email >

Core Services

We provide and maintain unique and modular platforms that enable multi-layered, comprehensive neurodevelopmental disease model phenotyping. The available assays provide measures of target engagement by pharmacological agents and enable necessary preclinical PK/PD studies once a therapeutic is deployed in any of the available models.

  • One dedicated room with 9 rotating towers that allocate up to 900 cages total for standard care. Each cage can house up to 5 mice and contains highly absorbent bedding, specialized nesting material and tunnels or shelters.
  • Reverse light/dark cycle room can house up to 300 cages (lights off from 10:00am to 10:00pm).
  • Enriched housing is available with wide range of manipulations of sensory, motor and social environment, such as running wheels, labyrinths, toys and increased social settings, with the ability to significantly impact the trajectory of brain development.
  • Housing in special environment to manipulate the visual experience (dark-rearing) or sound exposure (white noise, selective frequency exposure, music exposure).

The IB&P allows precise and reproducible measurements of behavioral phenotypes of standard strains as well as of transgenic, knockout, genetically modified animal models, and rodent models of early life brain injury.

  • Neurobehavioral phenotyping with the SHIRPA test
  • Gait analysis is measured on a treadmill by video analysis of footprints using Digigait
  • Spatial cognition assessment through Morris water maze and Y-maze tests
  • Social recognition is evaluated using the 3-chamber test
  • Working memory and cognitive flexibility are assessed using novel object/subject recognition, Y maze and water T maze
  • Cognitive age-dependent flexibility is tested using foraging task
  • 12 operant touchscreens test working memory (2 choice discrimination), flexibility (reversal of previous rule learning), attention (5 choice serial reaction time) and motivation (progressive ratio)
  • Pain sensitivity to mechanical, heat and cold stimuli are tested measuring a wide range of voluntary reactions
Equipment – Behavior Manufacturer Functionality Pricing Location
Inframot (infrared motion sensor) tse-systems Voluntary activity $00 Karp 04016
Activity wheels tse-systems Voluntary activity $00 Karp 04016
Optomotor Cerebral Mechanics Visual acuity (brain stem reflex) $00 Karp 04004
qOMR (Automated Optomotor) Phenosys Visual acuity (brain stem reflex) $00 Karp 04004
Acoustic Startle Reflex Kinder Scientific Hearing reflex $00 Karp 04006
Pre-pulse Inhibition Kinder Scientific Sensorimotor gating $00 Karp 04006
Rotarod Columbus Instrument Ataxia $00 Karp 04018
Treadmill IITC Life Science Forced activity $00 Karp 04018
DigiGait Mouse Specifics Gait analysis $00 Karp 04004
Grip Strength tse-systems Motor strengh $00 Karp 04009
ActiTrack locomotor activity Harvard Apparatus Voluntary activity $00 Karp 04016
Conditioned Fear Ugo Basile Anxiety/Cognition $00 Karp 04006
Light Dark Box Ethovision XT (Noldus) Anxiety $00 Karp 04014
3-Chamber Sociability Test Ethovision XT (Noldus) Anxiety/Social Recognition $00 Karp 04014
Elevated Plus Maze Ethovision XT (Noldus) Anxiety $00 Karp 04014
Conditioned Place Preference Ethovision XT (Noldus) Emotional response to stimulus/condition $00 Karp 04014
Forced Swim Ethovision XT (Noldus) Response to Stress $00 Karp 04016
Tail Suspension Ethovision XT (Noldus) Response to Stress $00 Karp 04016
Open field/holeboards Kinder Scientific Anxiety/Activity $00 Karp 04016
Ultrasonic Vocalizations Sonotrack, Metris Communication $00 Karp 04016
Contextual Fear Conditioning Stoelting (ANY-maze) Anxiety/Cognition $00 Karp 04006
Novel Object Recognition Ethovision XT (Noldus) Short term Working memory $00 Karp 04014
Y Maze Ethovision XT (Noldus) Spatial short term working memory $00 Karp 04014
Water T Maze Ethovision XT (Noldus) Cognitive flexibility $00 Karp 04014
Morris Water Maze Ethovision XT (Noldus) Spatial short term working memory $00 Karp 04008
Operant Touchscreens Lafayette Instruments Attention/working memory/flexibility $00 Karp 04015
Hot plate Bioseb Noxious heat sensitivity $00 Karp 04016
Thermal Gradient
Bioseb Threshold heat preference $00 Karp 04016
Thermal place preference
Bioseb Threshold heat preference $00 Karp 04016
Von Frey Stoelting Mechanical allodynia threshold $00 Karp 04009

For specific information on core pricing, including industry pricing please contact Nathaniel Hodgson >

  • Multi-cage wireless video EEG and related bio-signals such as electrocardiography, core temperature, electromyography and actigraphy, for the identification of epileptic seizures, sleep stages, and effects of drugs, injury, genetic manipulation or other factors on a range of brain and body rhythms.
  • Wired EEG via mice head connection (if mice are too small or young for implants of the telemeters).
  • The preclinical transcranial magnetic stimulation (TMS) enables measures of cortical excitability and plasticity that are valuable as metrics of pharmaceutical target engagement, and also enables deeper electrophysiological phenotyping of rodent disease CNS models.
  • In vivo calcium imaging by multifiber photometry coupled with behavioral analysis such as operant behavior, social or anxiety tests;
  • Whole-body plethysmography system for measuring breathing function in conscious, unrestrained mice;
  • ECGenie system for measuring the electrocardiogram of conscious, unrestrained mice.
  • In vivo microdialysis for sampling neurochemicals and other constituents of the peripheral and central nervous systems in a conscious, freely moving rodent.
  • Open flow microperfusion (OFM) provides dynamic substance profiles and absolute substance quantification in the investigated target tissues and can currently be performed in brain and skin/adipose tissue, with anesthetized or awake and behaving animals.

The combination of traditional microdialysis and OFM allows core users to measure analytes ranging from small molecules to proteins and beyond to extracellular vesicles and infiltrating cells. This capability aids our users in understanding behavior at the neurochemical level.

  • Using HPLC and UV-vis, florescent and electrochemical detectors, we can isolate and measure compounds and proteins of interest.
  • In addition to the capacity for recording behavioral and electrophysiologic data from any mouse and rat strain, the IB&P Core offers users access to expertise in several epilepsy and traumatic brain injury models as follows:
    1. Acute chemoconvulsant: pentylenetetrazol, kainate, pilocarpine.
    2. Acquired epilepsy: fluid percussion traumatic brain injury, perinatal hypoxia/ischemia (by carotid ligation), kainite or pilocarpine status epilepticus (separate from acute chemoconvulsant models)
    3. Closed head injury concussion model: by single or repeated weight drop
Environmental Modifications BCH/IDDRC External Academic Location Instrument Details
Reversed lighting (in-house) $56 for 1 week $75 for 1 week
Fragmented Care (in-house) $56 for 1 week $75 for 1 week Karp 04013
Acoustic rearing (IAT)  $56 for 1 week  $75 for 1 week Karp 04013
Dark rearing (Plastic Concepts) $56 for 1 week $75 for 1 week Karp 04013
Enriched Housing (Viewpoint Lifesciences) $56 for 1 week $75 for 1 week Karp 04013

 

EEG Wireless (DSI) BCH/IDDRC External Academic Location Instrument Details
Surgery, dosing and post-op care (per in-house bred animal) $334.00 $584.50 Karp 04013 Learn More >
Neuromonitoring (per day/cage) $56.34 $98.60 Karp 04013
Seizure susceptibility testing (per animal) $120.90 $211.60 Karp 04013

 

EEG Tethered BCH/IDDRC External Academic Location Instrument Details
Tethered EEG, multichannel (Pinnacle) $22.50/hr Unassisted
$28.00 Assisted
$25.00/hr Unassisted
$34.00 Assisted
Karp 04013 Learn More >
Non-surgical EEG Subdermal wire electrode EEG (Cadwell) $22.50/hr Unassisted
$28.00 Assisted
$25.00/hr Unassisted
$34.00 Assisted
Karp 04013 Learn More >

 

Service BCH/IDDRC External Academic Location Instrument Details
Non-invasive Brain Stimulation
TMS with electromyography and/or accelerometry
$141.00 per animal $246.75 per animal CLS 14
Cardiovascular – ECGenie (Mouse Specifics) $22.50/hr Unassisted
$28.00 Assisted
$25.00/hr Unassisted
$34.00 Assisted
Karp 04009 Learn More >
Breathing – Whole Body Plethysmography (Scireq) $22.50/hr Unassisted
$28.00 Assisted
$25.00/hr Unassisted
$34.00 Assisted
Karp 04009
Pupillometry – In-house system $22.50/hr Unassisted
$28.00 Assisted
$25.00/hr Unassisted
$34.00 Assisted
Electroretinography (ERG) and visual evoked potential (VEP)
Electroretinography (Celeris)
$22.50/hr Unassisted
$28.00 Assisted
$25.00/hr Unassisted
$34.00 Assisted
  • Compound administration by intravenous, intraperitoneal, sub-cutaneous, oral, intra-muscular, intraventricular and enteric routes.
  • Collection of plasma and other body fluids as well as tissue samples for pharmacokinetic/pharmacodynamic measures and toxicology screens.
  • Stereotaxic surgery expertise to implant EEG/EMG electrodes, guide cannulae for subsequent drug administration, direct cranial injections and cranial windows.
  • Data analysis and metrics extraction either from data collected in the IB&P or from data sets provided by outside clients.
Service – Integrated Systems BCH/IDDRC External Academic Location Instrument Details
Calcium imaging and Optogenetics
Microendoscope (Mightex/OASIS) fitted with fiber-coupled laser and LED light sources that is used in conjunction with implantable gradient-index (GRIN) lenses
$22.50/hr Unassisted
$28.00 Assisted
$25.00/hr Unassisted
$34.00 Assisted
Karp 4 Portable Learn More >
Optogenetics
Multi-channel, programmable LED driver (Doric) and 473nm DPSS Laser system (Laserglow)
$22.50/hr Unassisted
$28.00 Assisted
$25.00/hr Unassisted
$34.00 Assisted
Karp 4 Portable
In vivo calcium imaging
Multi-fiber photometry (Neurophotometrics)
$22.50/hr Unassisted
$28.00 Assisted
$25.00/hr Unassisted
$34.00 Assisted
Karp 4 Portable
In vivo microdialysis
Raturn enabling freely moving assays (BASi)
$22.50/hr Unassisted
$28.00 Assisted
$25.00/hr Unassisted
$34.00 Assisted
Karp 04009 Learn More >

 

Service – Surgery BCH/IDDRC External Academic Location Instrument Details
Surgery
Stereotaxic frames, infusion pumps, microscopes, drills, anesthesia vaporizers and more
Free Unassisted
$50.00 Assisted
Free Unassisted
$60.00 Assisted
Karp 04002/17 Learn More >
  • Compound administration by intravenous, intraperitoneal, sub-cutaneous, oral, intra-muscular, intraventricular and enteric routes.
  • Collection of plasma and other body fluids as well as tissue samples for pharmacokinetic/pharmacodynamic measures and toxicology screens.
  • Stereotaxic surgery expertise to implant EEG/EMG electrodes, guide cannulae for subsequent drug administration, direct cranial injections and cranial windows.
  • Data analysis and metrics extraction either from data collected in the IB&P or from data sets provided by outside clients.
Service – Integrated Systems BCH/IDDRC External Academic Location Instrument Details
Calcium imaging and Optogenetics
Microendoscope (Mightex/OASIS) fitted with fiber-coupled laser and LED light sources that is used in conjunction with implantable gradient-index (GRIN) lenses
$22.50/hr Unassisted
$28.00 Assisted
$25.00/hr Unassisted
$34.00 Assisted
Karp 4 Portable Learn More >
Optogenetics
Multi-channel, programmable LED driver (Doric) and 473nm DPSS Laser system (Laserglow)
$22.50/hr Unassisted
$28.00 Assisted
$25.00/hr Unassisted
$34.00 Assisted
Karp 4 Portable
In vivo calcium imaging
Multi-fiber photometry (Neurophotometrics)
$22.50/hr Unassisted
$28.00 Assisted
$25.00/hr Unassisted
$34.00 Assisted
Karp 4 Portable
In vivo microdialysis
Raturn enabling freely moving assays (BASi)
$22.50/hr Unassisted
$28.00 Assisted
$25.00/hr Unassisted
$34.00 Assisted
Karp 04009 Learn More >

 

Service – Surgery BCH/IDDRC External Academic Location Instrument Details
Surgery
Stereotaxic frames, infusion pumps, microscopes, drills, anesthesia vaporizers and more
Free Unassisted
$50.00 Assisted
Free Unassisted
$60.00 Assisted
Karp 04002/17 Learn More >