Nanopore Sequencing Now Available at the Gene Analysis and Editing Core/Molecular Genetics Core!

We are excited to announce that Nanopore sequencing is now available as a full-service option at the Molecular Genetics Core. With the advanced capabilities of Oxford Nanopore Technologies’ PromethION 24, we now offer rapid, long-read sequencing for a broad range of ‘omics applications.

Nanopore Sequencing projects include Whole Genome/Transcriptome Sequencing that can:

• Capture native DNA and RNA Methylation information
• Sequence ultra-long DNA reads up to 3 MB and ultra-long RNA reads greater than 10 KB
• Identify twice as many Structural Variants as short-read WGS options
• Assemble highly contiguous genomes
• Phase heterozygous alleles without parental information
• Provide Rapid Turn-Around Times within a week
• Clinical-Grade Sequencing Depth with 10-75 kb length DNA and >99% Basecalling Accuracy with Ligation Sequencing Kit v14 Chemistry

Our Barcoding Kits can sequence over 500 plasmid and bacteria genomes with a single flow cell enabling the identification of low-abundance subpopulations!

If you’re interested in taking advantage of this new service, please reach out to the Core staff (catherine.brownstein@childrens.harvard.edu or nathaniel.edisis@childrens.harvard.edu) to discuss how we can support your next project or schedule a sample drop-off.