Human Neuron Unit
Boston Children’s Hospital’s Human Neuron Unit offers a new paradigm for disease modeling and preclinical drug screening in an academic setting. The mission of the core is to help establish human induced pluripotent stem cell (iPSC)-derived model systems for neurological disorders as well as provide support for preclinical research developing phenotypic assays and screens to identify novel therapeutic targets.
Staff
Contact Us
Elizabeth Buttermore, PhD
Director, Translational In Vitro Models
Location:
Center for Life Sciences, 3 Blackfan Circle, CLS 12160.1-Boston, MA 02115, CLS 12160.4, 12161, 12163, and 12121
HNC Services & Fees
The Human Neuron Differentiation Service provides well-characterized human iPSC- derived neurons to academic investigators and industry collaborators to support translational research programs.
To initiate these services, please contact Elizabeth Buttermore > And fill out the Service Request Form >
| Differentiation Service | Internal BCH | External Academic | External Industry |
| Project planning fee (per new project) | $290 | $377 | $493 |
| Access to control iPSC line (per line) | $382 | $497 | $650 |
| iPSC installation (per line, 5 vials) | $872 | $1,133 | $1,482 |
| iPSC expansion (per line, 20 vials) | $1,680 | $2,184 | $2,856 |
| 2D spinal motor neurons (2wo) (1 batch = two 10cm) | $1,640 | $2,131 | $2,788 |
| 2D spinal motor neurons (2wo, dissociated) (1 batch = two 10cm) | $1,857 | $2,415 | $3,157 |
| EB spinal motor neurons (4wo) (1 batch = two 10cm) | $1,455 | $1,892 | $2,474 |
| EB spinal motor neurons (4wo, dissociated) (1 batch = two 10cm) | $1,752 | $2,277 | $2,979 |
| Monolayer sensory neurons (8wo) (1 batch = two 10cm) | $1,726 | $2,245 | $2,936 |
| Monolayer sensory neurons (8wo, dissociated) (1 batch = two 10cm) | $1,945 | $2,528 | $3,306 |
| Monolayer cortical neurons (10wo) (1 batch = two 10cm) | $2,229 | $2,898 | $3,788 |
| NGN2 cortical neurons (1wo) (1 batch = two 10cm) | $931 | $1,211 | N/A |
| NGN2 cortical neurons (1wo, dissociated) (1 batch = two 10cm) | $1,138 | $1,478 | N/A |
| NGN2 cortical neurons (2wo) (1 batch = two 10cm) | $1,537 | $1,998 | N/A |
| NGN2 cortical neurons (3wo) (1 batch = two 10cm) | $1,796 | $2,334 | N/A |
| iGABA neurons (1wo, dissociated) (1 batch = two 10cm) | $1,189 | N/A | N/A |
| iGABA neurons (5wo) (1 batch = two 10cm) | $3,318 | N/A | N/A |
| iPSC maintenance (per line, per week) | $104 | $136 | $177 |
| MEA plating (reagents) ((1) 48-well plate) | $128 | $167 | $218 |
| MEA plating (labor) (1-3 plates) | $208 | $271 | $354 |
| MEA plating (labor) (4-6 plates) | $416 | $541 | $708 |
| MEA plating (labor) (7-10 plates) | $624 | $812 | $1,061 |
| MEA plate (1 plate) | $400 | $420 | $495 |
| MEA glia for plating (1-3 plates) | $625 | $625 | $625 |
| MEA glia for plating (4-6 plates) | $1,250 | $1,250 | $1,250 |
| MEA glia for plating (7-10 plates) | $1,875 | $1,875 | $1,875 |
| MEA media changing (reagents) (per plate for 1 week) | $156 | $203 | $266 |
| MEA media changing (labor) (per week) (1-3 plates) | $76 | $99 | $130 |
| MEA media changing (labor) (per week) (4-6 plates) | $152 | $198 | $259 |
| MEA media changing (labor) (per week) (7-10 plates) | $228 | $297 | $388 |
| High content imaging – plating (reagents) (per 1 96-well plate) | $58 | $76 | $99 |
| High content imaging – plating (labor) (1-3 plates) | $208 | $271 | $354 |
| High content imaging – plating (labor) (4-6 plates) | $416 | $541 | $708 |
| High content imaging – plating (labor) (7-10 plates) | $624 | $812 | $1,061 |
| High content imaging – media changing (reagents) (per plate) | $116 | $151 | $198 |
| High content imaging – media changing (labor) (per week) (1-3 plates) | $76 | $99 | $130 |
| High content imaging – media changing (labor) (per week) (4-6 plates) | $152 | $198 | $259 |
| High content imaging – media changing (labor) (per week) (7-10 plates) | $228 | $297 | $388 |
| IncuCyte analysis (per experiment) | $318 | $414 | $541 |
| MACS sorting (reagents) (per sample) | $48 | $63 | $82 |
| MACS sorting (labor) (per 2 samples) | $132 | $172 | $225 |
Service Request Form > Service Request – Sample Procurement > Service Request – iPSC >
The Phenotyping Service provides high content image analysis, multi-electrode array functional analyses, and targeted RNA sequencing of iPSC-derived neurons to help answer fundamental biological questions by establishing assays that screening.
To initiate these services, please contact Elizabeth Buttermore > And fill out the Service Request Form >
| Phenotyping Service | Internal BCH | External Academic | External Industry |
| Project planning fee (per new project) | $290 | $377 | $493 |
| MEA recording (per recording) | $49 | $64 | $84 |
| MEA data analysis (per plate) (multiple time points) | $439 | $571 | $747 |
| High content imaging – image capture (per plate) | $162 | $211 | $276 |
| High content imaging – data analysis (per experimental endpoint or algorithm) | $665 | $865 | $1,131 |
| Fixing and staining (reagents) (per plate) | $122 | $159 | $208 |
| Fixing and staining (labor) (1-3 plates) | $318 | $414 | $541 |
| Fixing and staining (labor) (4-6 plates) | $636 | $827 | $1,082 |
| Fixing and staining (labor) (7-10 plates) | $954 | $1,241 | $1,622 |
| Drug treatment dose response (per drug) | $289 | $376 | $492 |
| Drug treatment (one dose; one daily exchange) | $81 | $106 | $138 |
| IncuCyte imaging (per day) | $58 | $76 | $99 |
| IncuCyte analysis (per experiment) | $318 | $414 | $541 |
| MACS sorting (reagents) (per sample) | $48 | $63 | $82 |
| MACS sorting (labor) (per 2 samples) | $132 | $172 | $225 |
| LDH assay (reagents) (per sample) | $1.16 | $1.50 | $1.96 |
| LDH assay (labor) (per experiment) | $81 | $106 | $138 |
| Collection of cells for RNA extraction (1 batch of lines) | $81 | $106 | $138 |
| RNA extraction (reagents) (per sample) | $7 | $10 | $12 |
| RNA extraction (labor) (1-6 samples) | $243 | $316 | $414 |
| qPCR reagents (per sample) | $12 | $16 | $21 |
| qPCR reagents (per project additional costs; per taqman primer/probe purchased) | $87 | $114 | $148 |
| qPCR labor (per plate) | $162 | $211 | $276 |
| AmpliSeq targeted RNA Sequencing (per sample) | $315 | $410 | $536 |
Service Request Form > Service Request – Sample Procurement > Service Request – iPSC >
The expanded Assay Development and Screening Facility provides multiple live-cell screening platforms that can assess image- and activity-based measures of live neurons in a medium/high-throughput capacity. This facility also includes robotics and liquid handlers to improve the reproducibility of experiments across screening endeavors.
To initiate these services, please email Lee Barret >
| Instrument | Internal BCH | External Academic | External Industry |
| Molecular Devices ImageXpress Micro Confocal | $60/hr weekdays $30/hr nights & weekends |
Contact for Pricing | Contact for Pricing |
| IXM Analysis computer 1 | $10/hr | Contact for Pricing | Contact for Pricing |
| IXM Analysis computer 2 | $10/hr | Contact for Pricing | Contact for Pricing |
| IXM Analysis computer 3 | $10/hr | Contact for Pricing | Contact for Pricing |
| Spinnaker liquid handling cell automation system | $6.25/hr | Contact for Pricing | Contact for Pricing |
| ThermoFisher Arrayscan XTi | $45/hr weekday $22.50/hr nights & weekends |
Contact for Pricing | Contact for Pricing |
| Arrayscan Remote Disk Based Scan Access | $15/hr | Contact for Pricing | Contact for Pricing |
| Hamamatsu | $45/hr | Contact for Pricing | Contact for Pricing |
| Agilent Bravo liquid handler | $20/hr weekdays $10/hr nights & weekends |
Contact for Pricing | Contact for Pricing |
| Agilent Bravo liquid handler in screening room | $20/hr | Contact for Pricing | Contact for Pricing |
| Formulatrix Matrix Liquid Handler | $60/hr | Contact for Pricing | Contact for Pricing |
| Axion Maestro MEA | $20/hr | $26/hr | $34/hr |
| Axion MEA + CO2 | $38/hr | $44/hr | $52/hr |
| BSC hood | $15/hr | $18/hr | |
| Integra Viaflo 96/384 | $15/hr | $20/hr | $25/hr |
| IncuCyte S3 Live Cell Analysis | $10/hr | $13/hr | $17/hr |
| IncuCyte S3 Live Cell Imaging | $48/day | $66/day | $90/day |
| Echo Revolve Fluorescent Microscope | $15/hr | $17/hr | |
| Integra Voyager II Pipette 12-channel; 5-125ul | $10/hr | $13/hr | $17/hr |
| Integra Voyager II Pipette 8-channel; 10-300ul | $10/hr | $13/hr | $17/hr |