Overview

The IB&P allows precise and reproducible measurements of behavioral phenotypes of standard strains as well as of transgenic, knockout, genetically modified animal models, and rodent models of early life brain injury.

• Neurobehavioral phenotyping with the SHIRPA test
• Gait analysis is measured on a treadmill by video analysis of footprints using Digigait
• Spatial cognition assessment through Morris water maze and Y-maze tests
• Social recognition is evaluated using the 3-chamber test
• Working memory and cognitive flexibility are assessed using novel object/subject recognition, Y maze and water T maze
• Cognitive age-dependent flexibility is tested using foraging task
• 12 operant touchscreens test working memory (2 choice discrimination), flexibility (reversal of previous rule learning), attention (5 choice serial reaction time) and motivation (progressive ratio)
• Pain sensitivity to mechanical, heat and cold stimuli are tested measuring a wide range of voluntary reactions

For specific information on core pricing, including industry pricing please contact Nathaniel Hodgson >

• Multi-cage wireless video EEG and related bio-signals such as electrocardiography, core temperature, electromyography and actigraphy, for the identification of epileptic seizures, sleep stages, and effects of drugs, injury, genetic manipulation or other factors on a range of brain and body rhythms.
• Wired EEG via mice head connection (if mice are too small or young for implants of the telemeters).
• The preclinical transcranial magnetic stimulation (TMS) enables measures of cortical excitability and plasticity that are valuable as metrics of pharmaceutical target engagement, and also enables deeper electrophysiological phenotyping of rodent disease CNS models.
• In vivo calcium imaging by multifiber photometry coupled with behavioral analysis such as operant behavior, social or anxiety tests;
• Whole-body plethysmography system for measuring breathing function in conscious, unrestrained mice;
• ECGenie system for measuring the electrocardiogram of conscious, unrestrained mice.
• In vivo microdialysis for sampling neurochemicals and other constituents of the peripheral and central nervous systems in a conscious, freely moving rodent.
• Open flow microperfusion (OFM) provides dynamic substance profiles and absolute substance quantification in the investigated target tissues and can currently be performed in brain and skin/adipose tissue, with anesthetized or awake and behaving animals.

The combination of traditional microdialysis and OFM allows core users to measure analytes ranging from small molecules to proteins and beyond to extracellular vesicles and infiltrating cells. This capability aids our users in understanding behavior at the neurochemical level.

• Using HPLC and UV-vis, florescent and electrochemical detectors, we can isolate and measure compounds and proteins of interest.
• In addition to the capacity for recording behavioral and electrophysiologic data from any mouse and rat strain, the IB&P Core offers users access to expertise in several epilepsy and traumatic brain injury models as follows:
  1. Acute chemoconvulsant: pentylenetetrazol, kainate, pilocarpine.
  2. Acquired epilepsy: fluid percussion traumatic brain injury, perinatal hypoxia/ischemia (by carotid ligation), kainite or pilocarpine status epilepticus (separate from acute chemoconvulsant models)
  3. Closed head injury concussion model: by single or repeated weight drop

• Compound administration by intravenous, intraperitoneal, sub-cutaneous, oral, intra-muscular, intraventricular and enteric routes.
• Collection of plasma and other body fluids as well as tissue samples for pharmacokinetic/pharmacodynamic measures and toxicology screens.
• Stereotaxic surgery expertise to implant EEG/EMG electrodes, guide cannulae for subsequent drug administration, direct cranial injections and cranial windows.
• Data analysis and metrics extraction either from data collected in the IB&P or from data sets provided by outside clients.

• Compound administration by intravenous, intraperitoneal, sub-cutaneous, oral, intra-muscular, intraventricular and enteric routes.
• Collection of plasma and other body fluids as well as tissue samples for pharmacokinetic/pharmacodynamic measures and toxicology screens.
• Stereotaxic surgery expertise to implant EEG/EMG electrodes, guide cannulae for subsequent drug administration, direct cranial injections and cranial windows.
• Data analysis and metrics extraction either from data collected in the IB&P or from data sets provided by outside clients.